MEIC EVALUATION OF ACUTE SYSTEMIC TOXICITY. PART IV: IN VITRO RESULTS FROM 67 TOXICITY ASSAYS USED TO TEST REFERENCE CHEMICALS 31-50 AND A COMPARATIVE CYTOTOXICITY ANALYSIS

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Authors
  1. Clemedson, C.
  2. Andersson, M.
  3. Aoki, Y.
  4. Barile, F.A.
  5. Sawyer, T.W.
  6. and others
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);Cytotoxicology Lab, Uppsala (Sweden)
Abstract
Results from tests on the Mulitcentre Evaluation of In Vitro Cytotoxicity (MEIC) reference chemicals 31-50 in 67 different in vitro toxicity assays are presented in this paper as a prerequisite to in vitro/in vivo comparisons for all MEIC in vitro toxicity data in forthcoming papers, i.e. the final MEIC evaluation of the relevance of the tests. With the aim of increasing knowledge about the relative significance of some in vitro methodological factors, the strategies and methods of the preceding significance of some in vitro methodological factors, the strategies and methods of the preceding parts in the MEIC series (Parts II and III) were again employed to enable comparative cytotoxicity analysis of the new in vitro results presented in this paper. A principal components analysis (PCA) of the results from tests of the 20 chemicals in 67 assays demonstrated a dominating first component describing as much as 74% of the variance in the toxicity data, indicating a similar ranking of the cytotoxicities of the chemicals in most of the tests. The influence on the general variability of the results of a few, key methodological factors was also evaluated by using linear regression comparisons of the results of all pairs of methods available in the study, i.e. methods which were similar in all respects except for the factor being analysed. TRUNCATED
Keywords
Basal cytotoxicity;Comparative ceel toxicology;Differential cytotoxicity;In vitro toxicology;Multivariate analysis;Protein denaturation;Toxicity testing
Date of publication
01 Jun 1998
Number of Pages
53
Reprinted from
ATLA, vol 26, 1998, p 131-183
DSTKIM No
98-01567
CANDIS No
508388
Format(s):
Hardcopy;Document Image stored on Optical Disk

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