PROPHYLAXIS AGAINST LIPOPOLYSACCHARIDE-INDUCED ACUTE LUNG INJURY BY ALPHA-TOCOPHEROL LIPOSOMES

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Authors
  1. Suntres, Z.E.
  2. Shek, P.N.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
To investigate whether intravenously administered liposomal alpha-tocopherol can protect the lung from the injurious action of Escherichia coli lipopolysaccharide (LPS). Design: Prospective, randomized animal study. Setting: Government research laboratory. Subjects: Twenty adult male Sprague-Dawley rats. Interventions: Animals were intravenously pretreated with alpha-tocopherol liposomes (20 mg alpha-tocopherol/kg body weight), plain liposomes, or saline. Twenty-four hours later, pretreated animals were challenged with an intravenous injection of LPS (E. coli 0111:B4, 1 mg/kg body weight), and killed 2 hrs after LPS challenge. Measurements and Main Results: Challenge of saline-pretreated animals with LPS resulted in lung injuries as evidenced by an increase in wet lung weight and a reduction in pulmonary angio-tensin converting enzyme (25%) and alkaline phosphatase (28%), injury markers of lung endothealial and epithelial type II cells respectively. Also, LPS administration resulted in an increase in pulmonary myeloperoxidase and protease activities, indicative of a neutrophilic inflammatory response. TRUNCATED
Keywords
Lung injuries;Oxidant-induced tissue damage;Drug delivery;Neutrophilis;Oxidative stress;Vitamin E
Report Number
DCIEM-97-P-14 — Reprint
Date of publication
01 Feb 1998
Number of Pages
10
Reprinted from
Crit Care Med, vol 26, no 4, 1998, p 723-729
DSTKIM No
98-01929
CANDIS No
508777
Format(s):
Hardcopy;Document Image stored on Optical Disk

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