CHARACTERISATION OF BIOLOGICAL AEROSOL WITH A FLUORESCENT AERODYNAMIC PARTICLE SIZER IN OUTDOOR ENVIRONMENT

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Authors
  1. Ho, J.
  2. Spence, M.
  3. Hairston, P.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Biological aerosol measurement in real time is an urgent military requirement that also has many potential non-military applications. Such detection capabilities will be useful in environmental monitoring, for example, in gathering information in perceived hazardous areas like housing developments downwind of sewage treatment plants. Experience gained from measuring fluorescence signals of single bacterial spores under flow cytometry using UV excitation at 340-360 nm, was applied to concept testing of a prototype instrument, built to do the same for aersols. This machine was capable of resolving particle size as well as fluorescence intensity of each particle under laboratory and field conditions; it was called the fluorescent aerodynamic particle sizer (FLAPS). A second generation FLAPS (FLAPS2) was designed to be smaller, power efficient and field portable. FLAPS2 was challenged under refereed conditions in blind trials to determine if it could detect biological aerosols. This paper describes practical aspects of measuring biological aerosols when the results must be compared to reference samplers that provide culturable or "live" data. Treatment of particle size and fluorescence information is discussed with respect to FLAPS and reference data fidelity. Finally, an objective method is introduced to evaluate FLAPS data correlation to reference data. TRUNCATED
Keywords
Bio-aerosol detection;Fluorescence of Biological Aerosols;FLAPS (Fluorescent Aerodynamic Particle Sizer);Aerosol detection;Environmental monitoring;CB defence
Report Number
DRES-703 —
Date of publication
01 Jun 1998
Number of Pages
29
DSTKIM No
98-02276
CANDIS No
509141
Format(s):
Hardcopy;Document Image stored on Optical Disk

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