EFFECT OF RUTHENIUM RED ON VOLTAGE-SENSITIVE CA++ CHANNELS

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Authors
  1. Hamilton, M.G.
  2. Lundy, P.M.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
The organometallic dye, ruthenium red (RR) inhibited Ca++ influx, omega-conotoxin GVIA-sensitive Ca++ binding and Ca++ - dependent neurotransmitter release in a qualitatively similar manner in rat and chicken synaptosomes and in mammalian neuromuscular preparations, but had no effect on Ca++ - dependent processes mediated by the dihydropyridine-sensitive Ca++ channel. These effects are specific for the RR complex, as RuCI3 affected neither Ca++ influx, omega-conotoxin GVIA binding nor neurotransmitter release, but did, however, in contrast to RR, displace (3H)nitrendipine from synaptosomes. RR, in a manner similar to omega-conotoxin MVIIC and omega-agatoxin IVA (AgaIVA), also effectively inhibited the response of the rat diaphragm to nerve stimulation and blocked AgaIVA-sensitive Ca++ influx in the rat brain, suggesting a significant interaction at the P-type voltage-sensitive Ca++ channel. These effects of RR suggest that this amino complex affects both the N and the P domain of the Ca++ channel in the chicken brain and both the N- and P-type Ca++ channel which is intimately coupled to the Ca++ inlfux and neurotransmitter release in rat synaptosomes. TRUNCATED
Keywords
Agatoxin-IVA;Calcium channel inhibitors;Ca2+ translocation;Conotoxin-GVIA;C++ channels;Nerve terminals;Neurotransmitter release;P type calcium channels;Neurotran
Report Number
DRES-OL-93-012 — Reprint
Date of publication
03 Jan 1995
Number of Pages
10
Reprinted from
J of Pharmacology and Experimental Therapeutics, vol 273, no 2, 1995, p 940-947
DSTKIM No
98-02457
CANDIS No
509355
Format(s):
Hardcopy;Document Image stored on Optical Disk

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