THE ANTIGENICITY OF BACILLUS GLOBIGII SPORES

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Authors
  1. Lee, W.E.
  2. Thompson, G.
  3. Kokko, E.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Immunoassays of Bacillus globigii spores were carried out using a number of polyclonal antibody preparations. The limits of detection for the various antibody preparations gave similar values: all in the range of 1 ng or less. It was determined that, although spores reconstituted in distilled water could be detected in small amounts, the antibodies were directed at a soluble component in the samples, rather than the spres themselves. When the soluble component was separated from the spres by centrifugation, it was found that this component was responsible for over 90% of the immunoassay signal even though it composed less than 10% of the total weight. The nonreactivity of BG spores was also observed in transmission electron microscopy. Electron micrographs of the spores immunostained with colloidal gold did not indicate any appreciable antibody interaction.
Keywords
Thresholds;Immunoassay;Bacillus Globigii;Limits of detection;Antibody reactions
Report Number
DRES-M-1500 — Memorandum
Date of publication
01 Oct 1998
Number of Pages
34
DSTKIM No
98-02761
CANDIS No
509633
Format(s):
Hardcopy;Document Image stored on Optical Disk

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