IN VIVO MOUSE BONE MARROW MICRONUCLEUS ASSAY OF REACTIVE SKIN DECONTAMINATION LOTION

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Authors
  1. Mehta, R.D.
  2. Sawyer, T.W.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);Prairie Biological Research Ltd, Edmonton ALTA (CAN)
Abstract
The test article, Reactive Skin Decontamination Lotion, was tested in the in vivo mouse (Balb/cCr//Alt BM virus, antibody free) bone marrow micronucleus assay. The assay was conducted by exposing a test group of 10 mice (5 male and 5 female) to each of 5 doses of the test article; 2000, 1000, 500, 250, and 125 mg/kg. Sterile isotonic saline was used as the solvent and negative control. The test article was readily soluble at the highest dose. Mice were injected once traperitoneally and bone marrow samples were extracted 24 hours post-injection and smeared onto slides for analysis. The frequency of micronucleated polychromatic erythrocytes (PCE's) in the polychromatic erythrocyte population was used as the criterion for evaluating genotoxicity. At the dose levels tested, no apparent toxicity was observed. All the mice in treatment, control, and environmental groups were healthy during the course of the study. Cytotoxic effect of the treatment on the bone marrow cells was negligible as the percentage of PCE's among total erythrocytes at all dose levels tested was comparable with the negative control values. There was no statistically significant increase in the frequency of micronucleated polychromatic erythrocytes (PCE's) relative to the solvent control (p > 0.05) at the dose range tested. TRUNCATED
Keywords
Skin decontamination
Report Number
DRES-CR-98-32;PBR-970805-3 — Contractor Report (Final)
Date of publication
01 Jun 1998
Number of Pages
26
DSTKIM No
99-00234
CANDIS No
510057
Format(s):
Hardcopy;Document Image stored on Optical Disk

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