CHINESE HAMSTER OVARY CELL/HYPOXANTHINE-GUANINE PHOSPHORIBOSYL TRANSFERASE GENE MUTATION ASSAY FOR REACTIVE SKIN DECONTAMINATION LOTION

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Authors
  1. Mehta, R.D.
  2. Sawyer, T.W.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);Prairie Biological Research Ltd, Edmonton ALTA (CAN)
Abstract
The test article, Reactive Skin Decontamination Lotion, was tested for this ability to induce forward mutations conferring thioguanine resistant (TG-r) phenotype, at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus of the Chinese hamster ovary (CHO) cells. The cells of CHO-K1-BH4 cell line were exposed, both in the presence and absence of the metabolic activation system (S9), to six dose levels (156, 313, 625, 1250, 2500 and 5000 mu g/ml) of the test article. The test article was solubilized in the culture medium, alpha Minimum Essential Medium (without the serum and the antibiotics) and dimethylsulfoxide (DMSO) was used as the solvent for positive control chemicals, ethyl methanesculfonate (EMS) and benzo(a)pyrene (BaP). Cytotoxic effect of the test article was negligible at the dose range tested as relative clonal efficiency was greater than 70% in both, non-activated as well as S9-activated systems. The frequency of TG-r mutants in the negative control group was within the range of published solvent control values (0 - 20/E6 clonable cells). The positive controls, EMS and BaP produced significant (in excess of 15 and 20-fold increase, respectively) positive response for TG-r mutants over the solvent control values. Thus, the positive and negative controls fulfilled the requirements for a valid test. TRUNCATED
Keywords
Cytotoxicity
Report Number
DRES-CR-1999-009;STUDY-970805/4 — Contractor Report (Final)
Date of publication
01 Feb 1999
Number of Pages
33
DSTKIM No
99-00730
CANDIS No
510625
Format(s):
Hardcopy;Document Image stored on Optical Disk

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