Matrix-Assisted Laser Desorption Ionization (MALDI) Time-of-flight Mass Spectrometry (TOFMS) for Direct Analysis of Bioactive Peptide an Protein Mixtures

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Authors
  1. Li, L.
  2. D'Agostino, P.A.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);Alberta Univ, Edmonton ALTA (CAN) Dept of Chemistry
Abstract
The overall goal of this research was to develop a MALDI method of mixture analysis based on time-lag focusing matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS). The specific objectives were (1) to develop sample/matrix preparation methods for mixture analysis; and (2) to demonstrate the overall analytical performance of the MALDI method for mixture analysis. We have achieved these specific golas, as detailed in this report. We have shown that direct analysis of peptide and protein mixtures by MALDI-TOFMS is strongly dependent on the sample and matrix preparation. A two-layer sample preparation method is demonstrated to be very effective for analyzing complex mixtures. In this method, the first layer on the MALDI probe is the densely packed matrix microcrystals formed by fast solvent evaporation of a matrix solution. A mixture solution containing both matrix and smaple is then deposited onto the first layer to form uniform analyte/matrix micro-cocrystals. It is found that the addition of matrix to the second-layer sample solution proves to be critical in analyzing mixtures of peptides and proteins covering a broad mass range. The effect of solvent conditions for preparing the second-layer solution is discussed. TRUNCATED
Keywords
Bioactive peptides;Electrospray;Matrix Assisted Laser Desorption Ionization (MALDI);Toxic peptides
Report Number
DRES-CR-1999-064 — Contract Report
Date of publication
01 Jun 1999
Number of Pages
17
DSTKIM No
99-01415
CANDIS No
511291
Format(s):
Hardcopy;Document Image stored on Optical Disk

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