Electrochemiluminescence and Immunochromatographic Analysis of Samples Collected by the Canadian Integrated Biochemical Agent Detection System at Joint Field Trials IV

PDF

Authors
  1. Fulton, R.E.
  2. Stadnyk, L
  3. Spence, M.
  4. Ho, J.
  5. Boulet, C.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
This report documents the analysis of samples taken at Joint Field Trials (JFT) IV for two biological agent simulants, Bacillus subtilis var, niger ssp. globigii (BG) and Erwinia herbicola (EH), by two different identification technologies, electro-chemiluminescence immunoassay (ECLA) and immunochromatographic assay (ICA). In each of 25 trials, BG or EH was disseminated and then collected by one or more CIBADS collector systems, which consisted of two prototype CIBADS Sentry units located at different positions on the test grid and a Concept Demonstrator unit located outside the grid. On analysis of samples by ECLA, BG and EH were identified in 100% of trials in which BG and EH, respectively, were disseminated. The amount of simulant in samples were estimated from standard concentration curves. BG was found to be present in samples in concentrations ranging from approximately 7x10E4 to 7x10E6 cfu/ml; the concentraton of EH in samples ranged from approximately 1x10E3 to 1x10E4 cfu/ml. For a given collection period, the amount of simulant identified by ECLA in samples was positively correlated with the amount of simulant collected by referee samplers. TRUNCATED
Keywords
Bio-aerosol detection;Fluorescence of Biological Aerosols;Aerosol detection;CIBADS (Canadian Integrated Biological Agent Detection System);Electrochemiluminescence Analyzer;ECLA (Electrochemiluminescence Immunoassay);ILA (Immunochromatographic assay);Erwinia herbicola (EH)
Report Number
DRES-TR-1999-066 — Technical Report
Date of publication
01 Jun 1999
Number of Pages
56
DSTKIM No
99-01725
CANDIS No
511512
Format(s):
Hardcopy;Document Image stored on Optical Disk

Permanent link

Document 1 of 1

Date modified: