Analysis of Bacillus Globigii Spores Using the BioDetector

PDF

Authors
  1. Lee, W.E.
  2. Thompson, H.G.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
The Canadian Forces have a mandate to be able to operate under the threat of chemical or biological attack. An automated immunoassay instrument of providing rapid identification of biological agents was used to analyse laboratory and field samples containing the field trial simulant Bacillus globigii (BG) spores. The BioDetector instrument was developed for US Army Edgewood Research Development and Engineering Center by Environmental Technologies Group. It was an automated version of the Threshold system (Molecular Device Corp) employing filtration-capture immunoassays and a light-addressable potentiometric sensor (LAPS). The experiments presented herein demonstrated that the instrument was capable of determining the presence of BG spores in the liquid laboratory and field trials samples. The time required to carry out the assays was 15 min. The lower limits of detection were in range of 10 - 30 ng/mL. The instrument software provided a four level qualitative alarm for each sample: negative, low, medium and high. The principal limitations of the instrument were its large size and complicated fluidic network.
Keywords
LAP (Light Addressable Potentiometry);Immunoassay;Filtration-Capture Immunoassay;Biosensors;Bacillus globigii;BG spores
Report Number
DRES-TM-1999-154 — Technical Memorandum
Date of publication
01 Nov 1999
Number of Pages
29
DSTKIM No
CA000327
CANDIS No
512295
Format(s):
Hardcopy;Document Image stored on Optical Disk

Permanent link

Document 1 of 1

Date modified: