High Temperature Stress Induced Changes in Pseudomonas aeruginosa: Enzymology

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Authors
  1. Chaudhry, G.R.
  2. Bhatti, A.R.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);Oakland Univ, Rochester MI (US) Dept of Biological Sciences
Abstract
Growth of Pseudomonas aeruginosa at 46C is accompained by an almost complete inhibition of periplasm-located alkaline phosphatase (APase) and cyclic-21,3'-phosphodiesterase, whereas 5'nucleotidase and ribonuclease I (RNase 1) are only partially affected. Cytoplasmic membrane enzymes such as glucose and gluconate dehydrogenases and cytochromes a, b and c are partially affected by growth at 46C. When a culture grown at 37C is shifted to 46C, the cytochrome levels are decreased indicating that the high temperature inactivates the respective proteins. Growth of P. aeruginosa at 46C had virtually no effect on the cytoplasm-located enzymes such as glucose-6-phosphate which were examined. The apparent rates of deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and protein synthesis of 46C grown cells are similar to those of 37C cells, however, the data indicate that transport systems may be impaired in 46C cells. The synthesis of APase is not inhibited in 46C grown cells and experiments performed in the presence of chloramphenicol and trypsin suggest that APase is secreted to the periplasm as a heat labile monomer which is unable to dimerize to form active enzyme.
Keywords
Pseudomonas aeruginosa;Periplasm;Cytochrome;P.aeruginosa
Report Number
DRES-TM-1999-075 — Technical Memorandum
Date of publication
01 Oct 1999
Number of Pages
22
DSTKIM No
CA000852
CANDIS No
512613
Format(s):
Hardcopy;Document Image stored on Optical Disk

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