Changes in circulating lymphocyte subpopulations and mitogen-stimulated response in a rat infusion model of intra-abdominal infection


  1. Martineau, L.
  2. Shek, P.N.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
To describe the alterations in circulatin contrations of immune cells as well as in in vitro mitogen-stimulated response in a recently developed rat model of intra-abdominal infection. Design: Randomized, controlled study. Setting: Government research facility. Subjects: Male Sprague-Dawley rats. Infected animals received an intraperitoneal infusion of 6.0 x 10E8 colony forming units of Escherichia coli during 12 hrs, whereas control rats received a sterile inoculum. All experimental animals underwent laparotomy and peritoneal lavage at the end of the infusion period. Blood samples were obtained 12 hrs, 36 hrs, or 7 days after the onset of infusion. Splenocytes were concomitantly harvested and assayed for response to the mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and lipopolysaccharides, as well as for production of interleukin (IL)-2. Infected rats showed a marked leukopenia (-82% for 36 hrs), with leukocyte counts returning to normal at 7 days. They also developed a marked lumphocytepenia throughout the study; this was achieved through comparable reductions in circulating T and B cells. Con A responses in both groups were similar for 7 days. TRUNCATED
Sepsis;Cytokines;Infections;Inflammatory response;Peritonitis;Immune response;Bacterial infections;Interleukin;Intra-abdominal surgery;Animal model
Report Number
DCIEM-98-P-24 — Paper
Date of publication
01 Mar 2000
Number of Pages
Hardcopy;Document Image stored on Optical Disk

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