Development of high sensitivity capilarry electrophoresis fluorescence system for the detection of biological molecules


  1. Dovichi, N.J.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);Alberta Univ, Edmonton ALTA (CAN) Dept of Chemistry
Chemical and biological weapons fall into three classes. The first class consists of small, synthetic molecules, which are considered as the classic chemical warfare agent. The second class of agents consists of biological organisms, including bacteria and viruses, which are the classic biological warfare agents. The third class compounds consists of toxic proteins and peptides produced by biological organisms. This contract considered the analysis and detection of these mid-spectrum agents. There are two basic strategies for the detection and identification of the mid-spectrum agents. Antibody-based immunoassays form one class of assays. Thes assays rely on the highly specific reaction between the antibody and its antigen. The specificity of this reaction is both its strength and weakness. There is relatively little cross-reactivity of the reagent with other molecules, so that false-alarms are expected to be relatively rare. However, immunoassays suffer from two problems. First, a positive signal is observed only if an antibody is used that recognizes the antigen. Novel or unexpected mid-range agents are not recognized by the immunoassay. Second, the large number of antibodies needed to screen most possible agents presents a logistical hurdle in the field. TRUNCATED
Capillary Electrophoresis (CE);Laser Induced Fluorescence (LIF);CE-LIF
Report Number
DRES-CR-2000-158 — Contractor Report (Final)
Date of publication
01 Aug 2000
Number of Pages
Hardcopy;Document Image stored on Optical Disk

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