Automated microchip platform for immunuoassay analysis

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Authors
  1. Lee, W.E.
  2. Jemere, A.B.
  3. Attiya, S.
  4. Chiem, N.H.
  5. Paulson, M.
  6. and others
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
An instrument comprising the components and subsystems of an automated microfluidic chip-based platform for bioanalysis by immunoassay is described. The device uses micromachined glass plates for the fabrication of channel networks and combines electroosmotic pumping and capillary electrophoresis for fluid transport and separation with a miniature peristaltic pump for sample introduction. The complete system enables the chips to perform the key elements in analytical processing: sampling, injection, mixing, separation, detection, and elimination within about 3-5 min. We describe the design and subsystem configurations required to engineer a compact instrument box with an overall size of 30 x 35 x 50 cm. The instrument provides computer-based control of three peripheral subsystems incorporated into the box. These subsystems include high-voltage power supplies and relays for on-chip fluid control; a fluidic interface for transporting liquids to and from the chip; and a confocal, epiluminescent optical assembly for detection by laser-induced fluorescence using a solid-state diode laser source. The unit has a detection limit of 0.21 nM for cyanine 5 dye with a peak height variation of < + or - 3%. Short-term migration time variations of + or - 1%, varations of < + or - 3% over 14 days, and device-to-device varations of < + or - 3% were obtained. TRUNCATED
Keywords
Capillary Electrophoresis (CE);Laser Induced Fluorescence (LIF);Immunoassay separations;Microfluidics;Microchip;SIC (Sample Introduction Channel);PMT (Photomultiplier tube);PTFE (Polytetrafluoroethylene);Bioanalysis
Report Number
DRES-SL-1999-071 — Scientific Literature
Date of publication
21 Dec 2000
Number of Pages
12
Reprinted from
J. Cap. Elec, & Microchip Tech, vol 6, 1999, p 51-59
DSTKIM No
CA010760
CANDIS No
514974
Format(s):
Hardcopy;Document Image stored on Optical Disk

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