Exercise elevates plasma levels but not gene expression of IL-1 Beta, IL-6, and TNF-alpha in blood mononulcear cells


  1. Moldoveanu, A.I.
  2. Shepard, R.J.
  3. Shek, P.N.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Physical activity induces a subclinical inflammatory response, mediated in part by leukocytes, and manifested by elevated concentrations of circulating proinflammatory cytokines, including interleukin (IL)-1Beta, IL-6, and tumor necrosis factor-alpha (TNF-alpha). However, the source of the cytokines that appear during exercise remains unknown. In this study, we examined exercise-induced changes in plasma cytokine concentrations and their corresponding mRNA expression in peripheral blood mononuclear cells. Then healthy (peak oxygen uptake = 48.8 + or - 6.5 (SD) ml-kg 1(-).min 1(-) but untrained men (age = 25 + or - 5 (SD) yr) undertook 3 h of exercise (cycling and inclined walking) at 60-65% peak oxygen uptake. Circulating leukocyte subset counts were elevated during and 2 h postexercise but returned to normal within 24 h. Plasma concentrations of IL-1Beta, IL-6, and TNF-alpha peaked at the end of exercise and remained elevated at 2 h (IL-6) and up to 24 h (IL-1Beta and TNF-alpha) postexercise. Cytokine gene expression in circulating mononuclear cells was measured by using the reverse transcriptase-polymerase chain reaction; mRNA accumulation did not change with exercise. TRUNCATED
Interleukin-6;Immune function;Immune response;Exertion;Immunostimulating;TNF (Tumor Necrosis Factor);Interleukin-1beta;Cytokines
Report Number
DCIEM-SL-1999-120 — Scientific Literature
Date of publication
15 May 2000
Number of Pages
Reprinted from
J Appl Physiol, vol 89, 2000 p 1499-1504
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