Vector for the secretion of Western Equine Encephalitis antigen

PDF

Authors
  1. Monette, P.L.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);Animal Diseases Research Institute, Lethbridge AB (CAN)
Abstract
Western Equine Encephalitis (WEE) virus is a member of the Alphavirus genus in the family Togaviridae. The virus genome consists of (+) sense single-stranded RNA encapsidated within an enveloped virion vectored by mosquitoes. The virus can cause encephalitis and central nervous system (CNS) disorders in horses, humans, birds and other species. The fatality rate in humans, 3-7% is lower than that of the related alphavirus Eastern Equine Encephalitis (EEE) virus, but infections with WEE virus are sufficiently debilitating that the virus constitutes a potential biological warfare agent. Currently, immunodetection and immunotherapy offer the best protection against WEE virus, EEE virus and other biological warfare agents. Monoclonal antibody technology and the cloning of recombinant antibodies represent powerful tools for the detection and identification of alphaviruses, as well as for prophylaxis and treatment of alphavirus infections. In order to derive the maximum potential benefit from these approaches, a safe and reliable source of antigen is required for the characterization of antibodies. The conventional method for producing viral antigen consists of growing and purifying the virus, inactivating it, and then safety testing the product. TRUNCATED
Keywords
WEEV (Western Equine Encephalitis Virus);Genetic engineering;Recombinant antibody technology;Insect vector
Report Number
DRES-CR-2000-199 —
Date of publication
01 Oct 2000
Number of Pages
17
DSTKIM No
CA011566
CANDIS No
515050
Format(s):
Hardcopy;Document Image stored on Optical Disk

Permanent link

Document 1 of 1

Date modified: