Immunochromatographic assays for identification of biological agents – NATO SIBCA Exercise I


  1. Fulton, R.E.
  2. Stadnyk, L.L.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
In March 1999, the NATO Panel VII Subgroup on Sampling and Identificaton of Biological and Chemical Agents (SIBCA) conducted the first international training exercise on identification of biological agents. Eleven NATO national laboratories participated in the exercise: Canada, France, Germany (two laboratories), Hungary, Italy, Netherlands, Norway, Poland, United Kingdom, and United States. Participating laboratories were sent five sample unknowns, four of which contained biological agents and one blank containing buffer only. Participants were advised that biological agents could consist of any one of the following 10 gamma-irradiated organisms: Bacillus anthracis, Yersinia pestis, Vibrio cholerae, Venezuelan Equine Encephalitis (VEE) virus, Francisella tularensis, Brucella melitensis, Burkholderia mallei, Yellow Fever virus, Vaccinia virus, or Coxiella burnetii. The participating laboratory for Canada was the Defence Research Establishment Suffield (DRES). For the training exercise, DRES screened sample unknowns by two different antibody-based identification technologies, one of which was the immunochromatographic assay (ICA). ICAs for six of the 10 possible biological agents were available from one or both of two commercial sources and SIBCA samples were screened for these six agents. TRUNCATED

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SIBCA;BWA identification;Biological Warfare Agents;Francisella tularensis;Yersinia pestis;Vibrio cholerae;Burkholderia mallei;Training exercise;Immunochromatographic assay (ICA);Antibody-based hand-held test kit (HHTK);Antibody-based hand-held assay (HHA);Ticket;Lateral flow device
Report Number
DRES-TR-2000-154 — Technical Report
Date of publication
01 Sep 2000
Number of Pages
Hardcopy;Document Image stored on Optical Disk

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