Colorimetric enzyme-linked immunosorbant assays for identification of biological agents – NATO SIBCA Exercise II

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Authors
  1. Fulton, R.E.
  2. Stadnyk, L.L.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
In February 2000, the NATO Panel VII Subgroup on Sampling and Identification of Biological and Chemical Agents (SIBCA) conducted the second international training exercise on identification of biological agents. Twelve NATO/Partners for Peace national laboratories participated: Austria, Canada, France, Germany (Two laboratories), Italy, the Netherlands, Norway, Poland, Sweden, the United Kingdom, and the United States. Participant laboratories were sent six sample unknowns and were advised that samples could contain any one of the following gamma-irradiated organisms: Bacillus anthracis, Yersinia pestis, Brucella melitensis, Francisella tularensis, Vibrio cholerae, Barkholderia mallei, Venezuelan equine encephalitis (VEE) virus, vaccinia virus, Coxiella burnetti, or Yellow fever virus. In addition, two of the samples would also contain potential battlefield interferents i.e., burning vegetation or burning diesel fuel. For this exercise, DRES screened sample unknown using a number of different technologies, one of which was the enzyme-linked immunosorbant assay (ELISA). ELISAs were developed for all 10 possible biological agents and SIBCA trial samples were screened for the presence of these agents. TRUNCATED
Keywords
BWA identification;Biological Warfare Agents;Burkholderia mallei;ELISA (Enzyme linked immunosorbant assays);Francisella tularensis;SIBCA;Training exercise;Vibrio cholerae;Yersinia pestis;Yellow fever virus
Report Number
DRES-TR-2000-111 — Technical Report
Date of publication
01 Oct 2000
Number of Pages
56
DSTKIM No
CA010480
CANDIS No
515326
Format(s):
Hardcopy;Document Image stored on Optical Disk

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