Characterization of 21 strains of Bacillus anthracis

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Authors
  1. Kournikakis, B.
  2. Bateman, C.
  3. Cherwonogrodzky, J.W.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Twenty-one strains of Bacillus anthracis currently held in the culture collection at DRES were characterized by colonial morphology, antibiotic sensitivity and Biolog (TRADEMARK) metabolic identification profiles. Aside from Bacillus anthracis strain Vollum which appeared more "crusty" on blood plates, the other strains were similar, giving a characteristic "Greenland" appearance (rough edge, off-white to tan colouration). Most strains were resistant to ceftazidime, colistin, bacitracin, polymyxin B and sensitive to the other antibiotics tested, such as ciprofloxacin. Biolog (TRADEMARK) metabolic identification profiles were prepared for each of the 21 strains of Bacillus anthracis as part of the identification database being developed for the Defence Reference Center for Infectious Agents (DRCIA) a center of expertise for endemic and exotic diseases of concern to the Canadian Forces) at DRES. A representative strain (Sterne) of Bacillus anthracis was used to test the disinfectant sensitivity of both Bacillus anthracis vegetative cells and spores. Bacillus anthracis vegetative cells were sensitive to 1:100 and 1:10 diluted Savlon (TRADEMARK) as well as 1:100 and 1:10 diluted household bleach while the spores were only sensitive to 1:10 diluted household bleach.
Keywords
Antibiotic sensitivity;Biolog
Report Number
DRES-TM-2000-157 — Technical Report
Date of publication
01 Dec 2000
Number of Pages
39
DSTKIM No
CA010602
CANDIS No
515363
Format(s):
Hardcopy;Document Image stored on Optical Disk

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