Biological detector performance with a 402 nm laser diode


  1. Ho, J.
  2. Hairston, P.
  3. Spence, M.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);TSI Inc, St Paul MN (US)
In this paper, we report on our continuing attempts to improve on the FLAPS technology. The frequency tripled laser light source in FLAPS2 has been replaced by a small laser diode, with the potential to reduce cost by several orders of magnitude. The main focus of this paper will be to demonstrate that the instrument with a new light source performs similarly to previous generations of FLAPS. Laboratory and field trial data indicate that FLAPS performance using the two light sources gave very similar performance characteristics when presented with different biological aerosol simulants. Most encouraging was the similar fluorescence response from both excitation sources in the presence of spore aerosol; a material considered the most difficult to detect optically. Spores, by their physiological nature, contain very little biological material and the individual units are no larger than 0.7 Mum and enclosed by a very refractile spore coat. Due to this refractile characteristic, conventional light microscopy of spores reveal very little of its cellular content. In contrast, vegetative cells are better subjects for examination in that they can easily be stained. Thus, performance of biological detectors must be rated by their ability to measure spore aerosols and in these experiments, it has been shown that 10 ACPLA could be detected. TRUNCATED
Bio-aerosol detection;Fluorescence of Biological Aerosols;FLAPS (Fluorescent Aerodynamic Particle Sizer);Fluorescent Aerodynamic Particle Sizer;Aerosol detection;CB defence;Environmental monitoring
Report Number
DRES-TR-2000-190 — Technical Report
Date of publication
01 Jul 2001
Number of Pages
Hardcopy;Document Image stored on Optical Disk

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