Intracellular monocyte and serum cytokine expression is modulated by exhausting exercise and cold exposure

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Authors
  1. Rhind, S.G.
  2. Castellani, J.W.
  3. Brenner, I.K.M.
  4. Shephard, R.J.
  5. Zamecnik, J.
  6. and others
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
This study tested the hypothesis that exercise elicits monocytic cytokine expression and that prolonged cold exposure modulates such responses. Nine men (age, 24.6 + or - 3.8 y; VO2peak, 56.8 + or - 5.6 ml-kg1(1)) completed 7 days of exhausting exercise (aerobic, anaerobic, resistive) and underwent three cold, wet exposures (CW). CW trials comprised less than or equal to 6 h (six 1-h rest-work cycles) exposure to cold (5C, 20 km/h wind) and wet (5cm/n rain) conditions. Blood samples for the determination of intracellular and serum cytokine levels and circulating hormone concentrations were drawn at rest (0700), after exercise (difference 1130), and after CW (difference 2000). Whole blood was incubated with (stimulated) or without (spontaneous) lipopolysaccharide (LPS; 1 Mug/ml) and stained for CD14 monocyte surface antigens. Cell suspensions were strained for intracellular cytokine expression and analyzed by flow cytometry. The proportion of CD14+ monocytes exhibiting spontaneous and stimulated intracellular expression of interleukin (IL)-1Beta, IL-6, and tumor necrosis factor (TNF)-Alpha increased after exercise, but these cells produced less IL-1Beta and TNF-Alpha after CW when CW was preceded by exhausting exercise. TRUNCATED
Keywords
Catecholamines;Flow cytometry;Cytokines;Immune response;Cytotoxicity;Interleukin;Thermal stress;Cortisol
Report Number
DCIEM-SL-2000-069 — Paper
Date of publication
27 Feb 2001
Number of Pages
12
DSTKIM No
CA011749
CANDIS No
516310
Format(s):
Hardcopy;Document Image stored on Optical Disk

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