Capillary electrophoresis fractionation, enzyme chemistry, and mass spectrometry for sensitive detection and identification of peptides and proteins

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Authors
  1. Yeung, K.K-C.
  2. Kiceniuk, A.G.
  3. Keller, B.O.
  4. Li, L.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);Alberta Univ, Edmonton ALTA (CAN) Dept of Chemistry
Abstract
Detection and, particularly, identificaton of agents of biological origin (ABO) I.e., protein toxins, bacteria, and viruses) require sophisticated analytical techniques. Considering the chemical composition of these agents, it is clear that all ABOs contain peptides and proteins. Because of significant differences in genomes that govern the experession of peptides and proteins in different organisms, the identities of peptides and proteins are expected to be very different for different ABOs. Thus peptides and proteins can be used as biomarkers for ABO detection. An analytical technique capable of detection peptides and proteins with high sensitivity and specificity can potentially be used as a single platform for the detection of all ABOs. In the past decade or so, mass spectrometry has been rapidly advanced and it has now become the method of choice for peptide and protein analysis in many applications. Recently several research groups have proposed and illustrated the possibility of using MS for bacteria differentation. The potential advantages of MS for ABO detection and identification include high sensitivity, high specificity and relatively high speed. TRUNCATED
Keywords
Capillary Electrophoresis (CE);BWA identification
Report Number
DRES-CR-2001-144 — Contractor Report
Date of publication
01 Oct 2001
Number of Pages
53
DSTKIM No
CA020129
CANDIS No
516800
Format(s):
Hardcopy;Document Image stored on Optical Disk

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