Light addressable potentiometric immunoassays for identification of biological agents – NATO SIBCA Exercise I

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Authors
  1. Thompson, H.G.
  2. Fulton, R.E.
  3. Fisher, G.R.
  4. Stadnyk, L.L.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
In March 1999, the NATO Subgroup on Sampling and Identification of Biological and Chemical Agents (SIBCA) conducted the first international training exercise on identification of biological agents. Eleven NATO national laboratories participated: Canada, France, Germany (two laboratories), Hungary, Italy, Netherlands, Norway, Poland, United Kingdom, and United States. Participating laboratories were sent five sample unknowns, four of which contained biological agents and one blank contaiing buffer only. Participants were advised that biological biological agents could consist of any one of the following 10 cobalt-irradiated organisms: Bacillus anthracis, Yersinia pestis Vibrio cholerae, Venezuelan equine encephalitis virus (VEE), Francisella tularensis, Brucella melitensis, Burkholderia mallet, yellow fever virus, vaccina virus, or Coxiella burnetii. The participating laboratory for Canada was the Defence Research Establishment Suffield (DRES). For the training exercise, DRES screened sample unknown by two different antibody-based ientification technologies, one of which was the Threshold™ assay. Threshold™) assays for seven of the 10 possible biological agents were developed: Bacillus anthracis, Brucella melitensis, Francisella tularensis, Burkholderia mallei, Yersinia pestis VEE, and vaccinia virus.

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Keywords
BWA identification;Biological Warfare Agents;Burkholderia mallei;Francisella tularensis;LAP (Light Addressable Potentiometry);SIBCA;Training exercise;Vibrio cholerae;Yersinia pestis
Report Number
DRES-TR-2001-035 — Technical Report
Date of publication
01 Nov 2001
Number of Pages
36
DSTKIM No
CA020664
CANDIS No
517388
Format(s):
Hardcopy;Document Image stored on Optical Disk

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