Enzyme-Linked Immunosorbant Assays for Identification of Biological Agents in Sample Unknowns – NATO SIBCA Exercise III


  1. Fulton, R.E.
  2. Stadnyk, L.L.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
In February 2001, the NATO Panel VII Subgroup on Sampling and Identification of Biological and Chemical Agents (SIBCA) conducted the third international training exercise on identification of biological agents. Fourteen NATO/Partners for Peace national laboratories participated. Austria, Bulgaria, Canada, France, Germany (two laboratories), Italy, the Netherlands, Norway, Poland, Sweden, the United Kingdom, and the United States (two laboratories). The designated laboratory for Canada was the Defence Research Establishment Suffield (DRES). Participant laboratories were sent seven samples, four liquid (including one buffer blank) and three solid, consisting of soil. Participants were advised that samples could contain any one of the following gamma-irradiated organisms. Bacillus anthracis, Yersinia pestis, Brucella melitensis, Francisella tularensis, Vibrio cholerae, Burkholderia mallei, Venezuelan equine encephalitis (VEE) virus, Vaccinia virus, Coxiella burnetii, or Yellow fever virus. A number of immunologically-based technologies were used at DRES for screening of sample unknowns, one of which was the enzyme-linked immunosorbant assay (ELISA). Antigen capture ELISAs were developed for all 10 possible biological agents and were used to screen liquid samples, soil extracts, and a heterologous agent panel included as a control for specificity. TRUNCATED

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BWA identification;Biological Warfare Agents;Burkholderia mallei;Francisella tularensis;SIBCA;Vibrio cholerae;Yersinia pestis;Training exercise;ELISA (Enzyme linked immunosorbant assays)
Report Number
DRES-TR-2001-175 — Technical Report
Date of publication
01 Dec 2001
Number of Pages
Hardcopy;Document Image stored on Optical Disk

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