Proteomic Analysis of Bacillus Cereus Spores

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Authors
  1. Schwandt, K.E.
  2. Berger, B.J.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
Spores were harvested from Bacillus cereus 14579 grown in casitone-yeast medium for 4 days at 30C. Two-dimensional gel electrophoresis was performed on solubilized spore protein and twenty of the most abundant spots were analyzed by N-terminal amino acid sequencing. The identities of the N-terminal sequences obtained were determined by homology searching of the Genbank non-redundant database, and the B. anthracis and B. cereus genome projects. All of the identified proteins were plausible spore components, and included chaperonins, sporulation regulators, ribosomal proteins, proteases, and metabolic enzymes involved in energy production, radical detoxification, and germination. The conservation and extremely high identity of the identified proteins in both the B. cereus and B. anthracis genome databases confirmed the applicability of B. cereus spores as a surrogate model for the study of B. anthracis spores.

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Report Number
DRDC-SUFFIELD-TR-2002-149 — Technical Report
Date of publication
01 Dec 2002
Number of Pages
51
DSTKIM No
CA022250
CANDIS No
519124
Format(s):
Hardcopy;Document Image stored on Optical Disk

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