Pseudomonas Aeruginosa-Induced Lung Injury: Role of Oxidative Stress

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Authors
  1. Suntres, Z.E.
  2. Omri, A.
  3. Shek, P.N.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
Pseudomonas aeruginosa is a Gram-negative pathogen which can cause lung injury in immunocompromised patients, primarily by inducing a release of host-derived mediators responsible for the influx of phagocytes to the lung. These phagocytes exert their antimicrobial actions by releasing toxic metabolites, including reactive oxygen species and proteases, which can also cause cell injury. This study was carried out to assess the pulmonary oxidant-antioxidant status of male adult Sprague-Dawley rats infected with different numbers of P. aeruginosa (104- 107 CFU/animal). Intratracheal instillation of P. aeruginosa resulted in lung injury, as evidenced by increases in wet lung weight and decreases in the lung activities of angiotensin converting enzyme and alkaline phosphatase, enzymes localized primarily in pulmonary endothelial and alveolar type II epithelial cells, respectively. The P. aeruginosa-induced lung injury was directly related to the infiltration of neutrophils, as indicated by increases in myeloperoxidase activity. The challenge of animals with P. aeruginosa resulted in increases in lipid peroxidation and decreases in glutathione content which were associated with the indices of lung injury and neutrophil infiltration. Such a challenge also resulted in weakening the antioxidant defence system, as evidenced by decreases in superoxide dismutase, catalase and glutathione peroxidase activities. These data suggest that changes in the pulmonary oxidant-antioxidant status may
Keywords
Pseudomonas aeruginosa;Oxidative stress;Antioxidants;Lung injury;Pseudomonas aeruginosa;Oxidant-induced tissue damage;Lung injuries
Report Number
DCIEM-SL-2001-065 — Scientific Literature
Date of publication
01 May 2002
Number of Pages
8
DSTKIM No
CA023179
CANDIS No
520382
Format(s):
Electronic Document(PDF);CD ROM

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