Radiation Induced Bystander Effects in Human Lymphoblastoid Cells

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Authors
  1. Segura, T.M.
  2. Wilkinson, D.
  3. Prud'homme-Lalonde, L.
  4. Thorleifson, E.M.
  5. Lachapelle, S.
Corporate Authors
Defence R&D Canada - Ottawa, Ottawa ONT (CAN)
Abstract
It is well accepted that cells in response to radiation, may release transmissible factors. These transmissible factors, clastogenic factors, have been reported to induce genomic instability in cells that have not been directly exposed to radiation. We hypothesize that this observed by stander effect might be a consequence of cellular interactions via secretory proteins released by the irradiated cells to affect the non-irradiated cells and initiate a systemic stress response to deal with the exposure. TK6 cells, a human lymphoblastoid cell line with a stable karyotype (47 chromosomes) and a functional p53 protein, were chosen as the surrogate for determining the stress response activation. Non-irradiated TK6 cells were co-cultured with 1 Gy gamma-irradiated and non-irradiated TK6 cells in trans-walls, where the cells were kept separate but the culture media was free to diffuse across the membrane. Microarray analysis 8 hours post co-culturing monitored the gene expression changes and the dicentric assay was used to evaluate cytogenetic aberrations. Our findings show that this research model is an effective method of demonstrating the bystander effect using the dicentric assay and gamma-irradiated cells. From the cytogenetic results it is evident that a bystander effect can be seen, although chromosomal aberrations are more frequent in the irradiated samples compared to the bystander samples, the numbers seen in the bystander samples are significantly greater than those in th

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Keywords
Bystander effect;Clastogenetic factors;Biomarkers;Radiological emergency response;CBRN;Cytokines;Biological indicators;Biodosimetry;Gamma radiation
Report Number
DRDC-OTTAWA-TM-2003-104 — Technical Memorandum
Date of publication
01 Dec 2003
Number of Pages
42
DSTKIM No
CA023278
CANDIS No
520540
Format(s):
Hardcopy;CD ROM

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