Enzyme-Linked Immunosorbant Assays for Identification of Biological Agents in Sample Unknowns – NATO SIBCA Exercise IV

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Authors
  1. Thompson, H.G.
  2. Fulton, R.E.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
In January 2002, the NATO Panel VII Subgroup on Sampling and Identification of Biological and Chemical Agents (SIBCA) conducted the fourth international training exercise on identification of biological agents. Fourteen NATO/Partners for Peace national laboratories participated: Austria, Bulgaria, Canada, Denmark, France, Germany (two laboratories), Italy, the Netherlands, Norway, Poland, Sweden, the United Kingdom, and the United States. The designated laboratory for Canada was Defence R&D Canada – Suffield (DRDC Suffield). Participants laboratories were sent six swabs. Participants were advised that samples would contain any one of the following gamma-irradiated organisms: Bacillus anthracis, Yersinia pestis, Brucella melitensis, Francisella turarensis, Vibrio cholerae, Burkholderia mallei, Venezuelan equine encephalitis (VEE) virus, vaccinia virus, Coxiella burnetii, or yellow fever virus. A number of immunologically-based technologies were used at DRDC Suffield for screening of sample unknowns, one of which was the enzyme-linked immunosorbant assay (ELISA). Antigen capture ELISAs were developed for all 10 possible biological agents and were used to screen the samples and a heterologous agent panel included as a control for specificity. Five biological agent unknowns were identified by ELISA, three at the species level: B. melitensis, F. tularensis, and Y. pestis, and two at the genus level: Bacillus spp. One sample containing V. cholerae produced a false negative reacti

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Keywords
ELISA (Enzyme linked immunosorbant assays);Threshold™;Sproe stain;Gram stain
Report Number
DRDC-SUFFIELD-TR-2003-120 — Technical Report
Date of publication
01 Dec 2003
Number of Pages
53
DSTKIM No
CA023690
CANDIS No
521024
Format(s):
Hardcopy;Document Image stored on Optical Disk

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