An Improved Method for High-Efficiency Gene Delivery and Expression in Mammalian Cells

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Authors
  1. Wu, Q.J.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
Through millions of years of evolution, viruses have developed special molecular mechanism that allow them to bind to a host cell and to efficiently deliver their genetic material. To take advantage of these refined mechanisms, viruses have been modified as vectors to deliver desired genes into a large variety of cells. These include both in vitro (e.g. mammalian cell culture) and in vivo (e.g. mice) systems. To broaden DRDC’s existing programs in biotechnology, this report describes the development of an adenovirus-based vector for high efficiency gene delivery and expression in mammalian cells. To illustrate the feasibility of this vector system, a recombinant adenovirus vector was produced, which contains gene encoding the enhanced green fluorescent protein (EGFP). The robust expression of EGFP was demonstrated in cells infected with the recombinant adenovirus. The adenovirus vector described in this report can be applied to many research areas including: 1) the expression of recombinant proteins in tissue culture cells, 2) the development of live viral vectored vaccines, 3) the expression of therapeutic proteins (e.g. interferon and monoclonal antibody) and nucleic acids (e.g. small interfering RNAs, antisense RNA) in animals against pathogens, and 4) the production of polyclonal antibody by direct injection of animals with recombinant adenovirus expressing antigens.

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Report Number
DRDC-SUFFIELD-TR-2005-015 — Technical Report
Date of publication
01 Jan 2005
Number of Pages
32
DSTKIM No
CA025476
CANDIS No
523150
Format(s):
CD ROM

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