Avian Influenza Aerosol Sampling Campaign in the British Columbia Fraser Valley, 9-19 April 2004 – Sampling of rare biological events

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Authors
  1. Scholfield, L.
  2. Ho, J.
  3. Kournikakis, B.
  4. Booth, T.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
An outbreak of infectious Avian Influenza (AI) occurred during March-April 2004 in the Abbotsford area of British Columbia. There was a need to determine if infectious viral particles were being spread via an aerosol route. It was anticipated that such particles, should they be found, would occur as rare events. By coincidence, DRDC Suffield has been developing aerosol samplers that can be used to detect such rare events. Two sampling systems, consisting of a slit sampler array and a large column air sampler (XMX virtual impactor) were deployed. The sampling scheme was designed to capture four specific events requested by the CFIA. These were (1) up and down wind sampling of an acutely infected barn prior to euthanasia, (2) up and down wind sampling of a barn being euthanized to capture the displacement effect of carbon dioxide pushing air and possibly virus out the barn, (3) the effect of clearing the barn of carbon dioxide by starting up the ventilation fans after euthanasia, (4) the possible re-aerosolization of virus from a barn being actively composted or depopulated by sampling the up and down wind positions. In addition to these specific events, XMX samples were taken in 10 random positions in and around Abbotsford. The first two were in acutely infected barns with high morbidity/mortality while the remaining eight were randomly taken in the outbreak area. Liquid samples from an XMX device were analysed using PCR as the initial method of identification followed by viru

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Keywords
Avian flu;aerosol sampling;biological sampling;XMX sampler;slit array sampler;PCR;chickens,;British Columbia;Influenza
Report Number
DRDC-SUFFIELD-TM-2005-032 — Technical Memorandum
Date of publication
01 Mar 2005
Number of Pages
26
DSTKIM No
CA026100
CANDIS No
523877
Format(s):
CD ROM

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