Frontal Affinity Chromatography with Mass Spectrometry Detection for Probing Molecular Interactions – A New Mechanistic Approach for the Development of Detection & Identification and Medical Countermeasures against CB Agents

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Authors
  1. Chan, N.W.C.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
Frontal affinity chromatography with mass spectrometry detection (FAC-MS) is a powerful technique for the study of molecular interactions between receptors (proteins, antibodies, enzymes) and ligands (small molecules, antigens, drug compounds). Such interactions are fundamental to biochemical pathways, from the detection of a protein cascade involved in a disease state, to the study of the disease mechanisms, to high-throughput screening for the identification and optimization of lead compounds for drug discovery. FAC-MS is able to locate and characterize key molecular recognitions in complex biological systems that are otherwise "hidden” from current technologies. Thus, this cutting-edge technique can steer biotechnological research through key areas and eliminate many years of "hit-and-miss" experimentation. It will allow for rational design and development in areas, such as biodetection and identification, and medical countermeasure, which are currently of interest to the CF and DRDC. FAC-MS is amenable to high-throughput screening of large collections of compounds simultaneously. Currently, no other alternative method can provide unbiased, direct measures of binding affinity in a rapid and easy to perform manner as FAC-MS. This article outlines the plan of adapting FAC-MS from industry (Protana Inc.) to DRDC Suffield for defence research incorporating toxin research, drug discovery, recombinant antigenantibody optimization and proteomics.

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Keywords
Medical countermeasure;biological detection & identification;frontal affinity chromatography;mass;spectrometry;chemical and biological (CB) agents
Report Number
DRDC-SUFFIELD-TM-2005-173 — Technical Memorandum
Date of publication
01 Oct 2005
Number of Pages
32
DSTKIM No
CA026513
CANDIS No
524395
Format(s):
Hardcopy;Electronic Document(PDF)

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