Immunoassays for Identification of Biological Agents in Sample Unknowns – NATO SIBCA Exercise VI

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Authors
  1. Thompson, H.G.
  2. Fulton, R.E.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
In March 2005, the NATO Panel VII Subgroup on Sampling and Identification of Biological and Chemical Agents (SIBCA) conducted the sixth international training exercise on identification of biological agents. Fifteen biological sample unknowns, diluted in PBS, were sent to each laboratory and participants were advised that biological agents could consist of varying concentrations of any of the following three gamma-irradiated organisms: Bacillus anthracis, Yersinia pestis, or vaccinia virus. The object of the exercise was to assess each nation’s current capabilities in assay sensitivity for these agents. An immunological-based technology, the enzyme-linked immunosorbant assay (ELISA), was used at DRDC Suffield for screening of sample unknowns. Antigen capture ELISAs for each of the three possible biological agents were used to screen the samples. In addition, a fluorescent hand-held assay, the Rapid Analyte Measurement Platform (RAMP™), manufactured by Response Biomedical Corp., was used to analyze the samples for the presence of Bacillus anthracis and vaccinia virus. Five biological agent unknowns were identified by ELISA in the SIBCA samples, three samples containing B. anthracis and two samples containing Y. pestis. Two biological agent unknowns were identified by RAMP™ in the SIBCA samples, both containing B. anthracis. No agents were identified in 11 of the SIBCA sample unknowns. A comparison of the immunoassay results with the identity of organisms in SIBCA sample

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Keywords
Rapid Analyte Measurement Platform;RAMPTM;SIBCA Exercise VI;Biological Agents;ELISA (Enzyme linked immunosorbant assays)
Report Number
DRDC-SUFFIELD-TM-2005-223 — Technical Memorandum
Date of publication
01 Dec 2005
Number of Pages
40
DSTKIM No
CA026818
CANDIS No
524781
Format(s):
Hardcopy;Electronic Document(PDF)

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