Capillary Electrophoresis Laser-Induced Fluorescence for Screening Combinatorial Peptide Libraries in Assays of Botulinum Neurotoxin A

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Authors
  1. Laing, T.D.
  2. Marenco, A.J.
  3. Moore, D.M.
  4. Moore, G.J.
  5. Mah, D.C.W.
  6. Lee, W.E.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
Botulinum neurotoxin serotype A (BoNT/A) is a proteolytic enzyme muscle paralysis. It is a cause of food poisoning, a potential bioterrorist threat and, in low doses an emerging pharmaceutical product. No effective treatment is currently available for BoNT intoxication. Previously we developed a BoNT/A light chain enzyme assay using a peptide substrate based on the SNAP-25 protein target, with HPLC separation and UV detection of assay products, and applied the method to screen combinatorial peptide libraries for inhibitory activity to BoNT/A. We now report on development of a capillary electrophoresis laser-induced fluorescence (CE-LIF) method for measuring BoNT/A activity.
Date of publication
20 Oct 2005
Number of Pages
7
Reprinted from
Journal of Chromatography B, 843, 2006, p 240-246
DSTKIM No
CA028706
CANDIS No
526792
Format(s):
Hardcopy;Document Image stored on Optical Disk

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