Development and Characterization of a Suspension Bead Array Immunoassay for Ovalbumin

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Authors
  1. Snodgrass, M.
  2. Dickinson, L.T.
  3. Mah, D.C,
  4. Lam, V.
  5. Aw, C.
  6. Bhogal, H.S.
  7. Fulton, R.E.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN);Canada West Biosciences, Calgary ALTA (CAN)
Abstract
The Suspension Array System (SAS) has enabled the development of assays that are rapid, sensitive and specific for any biological threat agent. In this study a SAS immunoassay was developed and characterized for the toxin simulant ovalbumin. Optimization of this assay and its performance are presented. Results obtained were compared with data obtained by ELISA. The limit of detection (LOD) for the SAS ovalbumin assay was 4.88 ng/mL, compared to a LOD of 9.5 pg/mL for the ovalbumin ELISA. When compared to the LODs for ovalbumin reported in a previous study, the difference between the assay sensitivities was considerably smaller, with an ELISA LOD of 300 pg/mL and a SAS assay LOD of 1 ng/mL. The 500-fold sensitivity difference was likely a reflection of the longer incubation times for the ELISA versus the SAS assays, where the incubation time for the ELISA was 1 hour compared to an incubation time of 30 minutes for the SAS. The trade off for this decrease in sensitivity is a rapid assay that can be performed in half the time as a traditional ELISA. The SAS ovalbumin assay had a well-to-well variance of 5.56%, while its plate-to-plate reproducibility was 14.2%. In comparison, the ELISA ovalbumin assay had a well-to-well variance of 4.90%, whereas its plate-to-plate reproducibility was 14.8%. In summary, an immunological assay that was rapid, sensitive and reproducible was created for a biological threat simulant by suspension array technology. The procedures used to develop the
Report Number
DRDC-SUFFIELD-CR-2006-248 — Contractor Report
Date of publication
01 Dec 2006
Number of Pages
32
DSTKIM No
CA028851
CANDIS No
527022
Format(s):
CD ROM

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