Expression of the Envelope Proteins of Western Equine Encephalitis Virus by Adenovirus Vectors


  1. Wu, J.Q.H.
  2. Chau, D.
  3. Wong, C.
  4. Hu, W.-G.
  5. McCullough, C.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Western equine encephalitis (WEE) virus is a potential biological warfare agent. The virus is mainly endemic in the western regions of Canada and the United States as well as Central and South America. Medical countermeasures against WEE virus are currently lacking. There are no commercial vaccines or anti-viral drugs available to protect Canadian Forces (CF) personnel at risk. We propose to develop WEE vaccine based on human-adenovirusserotype- 5 (HAd5) vectors expressing the structural proteins of WEE virus. To this end, this report describes the construction and characterization of a HAd5 vector expressing the envelope proteins of the 71V-1658 strain of WEE virus. The vector designated as Ad5- WEEV was generated by the transfection of 293 cells with an infectious HAd5 clone that contains the gene encoding the E3-E2-6K-E1 polyprotein of 71V-1658. The presence of the E3-E2-6K-E1 gene in Ad5-WEEV was confirmed by polymerase chain reaction (PCR). The expression of E1 and E2 envelope proteins from Ad5-WEEV was detected by Western blot using monoclonal antibody (MAb) 11D2 specific for E1 and MAb 3F3 specific for E2. The results from this report demonstrate the feasibility of HAd5 vectors as a platform for the expression of viral proteins of WEE virus and lay a foundation for further testing HAd5- vectored WEE vaccines in animals.

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Report Number
DRDC-SUFFIELD-TR-2007-082 — Technical Report
Date of publication
01 Aug 2007
Number of Pages

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