Molecular Typing of Bacillus Anthracis using Multiple-Locus Variable Number Tandem Repeat Analysis (MLVA-8)

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Authors
  1. Stratilo, C.W.
  2. Bader, D.E.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
Bacillus anthracis, the etiological agent of anthrax, lacks character homoplasy and is genetically very monomorphic. Variable number tandem repeats (VNTR) are rapidly evolving stretches of DNA that can serve as markers for strain differentiation (molecular typing). A collection of B. anthracis strains were characterized using multiple-locus VNTR analysis (MLVA). MLVA allowed these strains to be placed within a phylogenetic framework. Several strains were discriminated from each other; however, some isolates were indistinguishable including a group of nine Canadian isolates. This work allowed verification of strain designation within the DRDC Suffield collection and allowed for the development of a high throughput genetic typing capability at DRDC Suffield.

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Keywords
Bacillus anthracis;molecular typing;MLVA;VNTR;Biological strains
Report Number
DRDC-SUFFIELD-TM-2007-282 — Technical Memorandum
Date of publication
01 Dec 2007
Number of Pages
26
DSTKIM No
CA030839
CANDIS No
529466
Format(s):
CD ROM

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