Fluorogenic Hand-held Immunoassay for Detection and Identification of Anthrax – Preliminary Evaluation of the Rapid Analyte Measurement Platform

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Authors
  1. Fulton, R.E.
  2. Barabé, N.D.
  3. Stadnyk, L.L.
  4. Negrych, L.M.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
The Rapid Analyte Measurement Platform (RAMP™) hand-held assay (HHA) system was evaluated for sensitivity and specificity in detection of B. anthracis by challenging with killed and live B. anthracis and closely related Bacillus strains in the BSL2 and BSL3 laboratories at DRDC Suffield. The limit of detection (LOD) of RAMP™ HHAs for detection of killed B. anthracis was 2.6 x 10E6 cfu/mL, corresponding to 2.6 x 10E4 cfu per test. Live B. anthracis (washed, heat shocked) and crude B. anthracis (not washed or heat shocked) were detected at 3.1 x 10E5 cfu/mL and 7.5 x 10E4 cfu/mL, respectively; these values correspond to a LOD per test of 3.1 x 10E3 cfu and 7.5 x 10E2 cfu, respectively. RAMP™ anthrax HHAs did not cross-react with other, closely related Bacillus strains at the concentrations examined, with the exception of B. globigii, which cross-reacted at a concentration in excess of 2.0 x 10E9 cfu/mL. The RAMP™ system may be a valuable alternative to chromogenic HHAs for rapid, on-site detection of B. anthracis by First Responders and military personnel.

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Report Number
DRDC-SUFFIELD-TM-2006-250 — Technical Memorandum
Date of publication
01 Dec 2006
Number of Pages
30
DSTKIM No
CA031355
CANDIS No
530201
Format(s):
CD ROM

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