Cytokine Profiling of Mice Vaccinated with Brucella Polysaccharide

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Authors
  1. Bhogal, H.S.
  2. McLaws, L.J.
  3. Russell, M.L.
  4. Barabe, N.D.
  5. Cherwonogrodzky, J.W.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
Although Brucella infection is one of the most common infections acquired in the laboratory, infection could also occur as a result of a deliberate act. The potential for Brucella to be weaponized and used as a biological warfare agent is a real concern as it is readily obtained and aerosolized, the infective dose is low, and can lead to a long-lasting disease. Therefore, there is a need for the development of an effective vaccine. A subcellular polysaccharide vaccine derived from Brucella suis has been shown to be effective in providing long-term protection from brucellosis in animal studies. The mechanism of action of this subcellular vaccine is unknown. In this study, we report the findings from cytokine profiling of mice injected with subcellular polysaccharide vaccine. A minor increase in IFN-γ and a depressed level of IL-5, coupled with a significant increase in TNF-α,hint at the induction of a Th1 response. Other cytokines such as IL-2, IL-4, IL-10, IL-12, and GM-CSF remain unchanged in response to vaccination with the polysaccharide. Although the polysaccharide may induce a cell-mediated response, this immune response is likely short-lived and, therefore, may not contribute to the long-term protection afforded by this subcellular vaccine.

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Report Number
DRDC-SUFFIELD-TM-2007-262 — Technical Memorandum
Date of publication
01 Dec 2007
Number of Pages
28
DSTKIM No
CA031397
CANDIS No
530288
Format(s):
CD ROM

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