Interferon-gamma gene delivery protects mice from the lethal infection of western equine encephalitis virus


  1. Wu, J.Q.H.
  2. Barabé, N.
  3. Nicole, D.
  4. Chau, C.
  5. Sha, A.
  6. Marshall, A.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Western equine encephalitis virus (WEEV) is a potential biological and bioterrorism agent.The aerosolized virus is easily transmitted to humans. Single-dose, fast-acting vaccines and therapeutics are urgently needed to provide rapid protection against WEEV in the event of bioterrorism attack or biowarfare. We found previously that a single dose injection of interferon alpha (IFN-α) expressed from human adenovirus type 5 (HAd5) vector rapidly and completely protects mice against WEEV. This result prompted us to investigate whether a single-dose injection of interferon-gamma (IFN-γ) expressed from HAd5 vector would also offer protection against WEEV in mice. A replication-deficient HAd5 vector (Ad5-mIFNγ)expressing mouse IFN-γ (mIFN-γ) was constructed. A single-dose injection of mice with Ad5-mIFNγ produced a high level of serum mIFN-γ. The production of mIFN-γ in mice was rapid, which was detected as early as 9 h after injection. A single-dose injection of Ad5-mIFNγ either before or immediately after WEEV challenge delayed the progression of WEEV infection and extended the time of survival. However, a single-dose injection of Ad5-mIFNγ did not improve the overall survival rate of the challenged mice. Thus, a single-dose injection of mIFN-γ expressed from HAd5 vector offers partial protection against the lethal infection of WEEV in mice. This work is part of an ongoing Technology Investment Fund(TIF) project of DRDC entitle

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Report Number
DRDC-SUFFIELD-TR-2008-224 — Technical Report
Date of publication
01 Dec 2008
Number of Pages

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