Interferon-gamma gene delivery protects mice from the lethal infection of western equine encephalitis virus

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Authors
  1. Wu, J.Q.H.
  2. Barabé, N.
  3. Nicole, D.
  4. Chau, C.
  5. Sha, A.
  6. Marshall, A.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
Western equine encephalitis virus (WEEV) is a potential biological and bioterrorism agent.The aerosolized virus is easily transmitted to humans. Single-dose, fast-acting vaccines and therapeutics are urgently needed to provide rapid protection against WEEV in the event of bioterrorism attack or biowarfare. We found previously that a single dose injection of interferon alpha (IFN-α) expressed from human adenovirus type 5 (HAd5) vector rapidly and completely protects mice against WEEV. This result prompted us to investigate whether a single-dose injection of interferon-gamma (IFN-γ) expressed from HAd5 vector would also offer protection against WEEV in mice. A replication-deficient HAd5 vector (Ad5-mIFNγ)expressing mouse IFN-γ (mIFN-γ) was constructed. A single-dose injection of mice with Ad5-mIFNγ produced a high level of serum mIFN-γ. The production of mIFN-γ in mice was rapid, which was detected as early as 9 h after injection. A single-dose injection of Ad5-mIFNγ either before or immediately after WEEV challenge delayed the progression of WEEV infection and extended the time of survival. However, a single-dose injection of Ad5-mIFNγ did not improve the overall survival rate of the challenged mice. Thus, a single-dose injection of mIFN-γ expressed from HAd5 vector offers partial protection against the lethal infection of WEEV in mice. This work is part of an ongoing Technology Investment Fund(TIF) project of DRDC entitle

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Report Number
DRDC-SUFFIELD-TR-2008-224 — Technical Report
Date of publication
01 Dec 2008
Number of Pages
31
DSTKIM No
CA032193
CANDIS No
531267
Format(s):
CD ROM

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