LIPOSOMES ARE EFFECTIVE CARRIERS FOR THE OCULAR DELIVERY OF PROPHYLACTICS

Authors
  1. Shek, P.N.
  2. Barber, R.F.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
Liposomes containing acetylcholinesterase were prepared by the freeze-drying method. The multilamellar morphology of the vesicles was revealed by freeze-fracture electron microscopy and their size distribution was determined by quasi-elastic light scattering. The vesicle diameters were in the range of about 0.2-4.0 mu m. The liposome preparations were tested for their ocular delivery of an entrapped cholinesterase enzyme in counteracting the miotic effect of diisopropylfluorophosphate (DFP), a prototype of a family of organophosphate poisons. The topical application of the enzyme-containing liposomes to the rabbit eye was found to confer a significant level of protection against DFP-induced miosis. In comparing the prophylactic effectiveness of different enzyme-bearing liposomes, positively charged vesicles were found to be more effective than either neutral or negatively charged vesicles. TRUNCATED
Report Number
DCIEM-87-P-23 — Research Paper; Reprint
Date of publication
01 Jan 1987
Number of Pages
8
Reprinted from
Biochimica et Biophysica Acta, 1987, p 229-236
DSTKIM No
88-00099
CANDIS No
53344
Format(s):
Hardcopy;Originator's fiche received by DSIS

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