An Affymetryx Microarray Design for Microbial Genotyping

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Authors
  1. Ford, B.N.
  2. Bader, D.
  3. Shei, Y.
  4. Ruttan, C.
  5. Mah, D.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
There is an ongoing requirement for development of high-density or multiplex assays for detection or identification of microbes. There is also a need to develop assays or toolsets that can detect or identify microbial threats without prior knowledge of the target microbe(s) in a given sample. Indeed, some samples that contain no culturable material (e.g. viable but non-culturable cells) will nonetheless contain detectable DNA fragments which might be of value with respect to forensics or attribution of source. For many pathogenic microbes, various specific tests already exist, but there are few general methods wherein a single adaptable tool can be applied to multiple species or to previously uncharacterized organisms. The high-density DNA microarray has the potential to address many of these requirements and thus complements existing identification tools. The microarray platform has for example, the ability to detect microbial DNA that is not a perfect match to known genomic DNA sequences, thus making it possible to detect microbial variants that might otherwise be missed. In this report, the design and preliminary testing of a high-density DNA microarray for the purpose of microbial identification and detection is described.

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Report Number
DRDC-SUFFIELD-TM-2009-183 — Technical Memorandum
Date of publication
01 Oct 2009
Number of Pages
48
DSTKIM No
CA034107
CANDIS No
533557
Format(s):
Electronic Document(PDF)

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