Multiple Locus Variable Number Tandem Repeat Analysis of Francisella tularensis

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Authors
  1. Stratilo, C.W.
  2. Bader, D.E.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
Francisella tularensis, the causative agent of tularemia, is highly virulent with an extremely low infective dose. Although F. tularensis comprises four subspecies (tularensis, holarctica, mediasiatica, and novicida), the two most clinically important subspecies are the highly virulent F. tularensis tularensis (nearctica, biovar type A) and the moderately virulent F. tularensis holarctica (palaearctica, biovar type B). F. tularensis is considered a potential biological weapon and listed as a Category A select agent by the Centers for Disease Control (CDC). A PCR-based typing system that targets variable number tandem repeats (VNTR) at multiple loci (MLVA) can distinguish very closely related bacterial strains. In this report we describe the MLVA typing of 24 strains of F. tularensis in the DRDC Suffield collection using 25 VNTR loci. The DRDC Suffield strains, although quite heavily weighted towards F. tularensis holarctica (18 strains), do have representation from other subspecies including F. tularensis tularensis A.I, F. tularensis tularensis A.II, F. tularensis holarctica B.V Japan; and F. tularensis mediasiatica.

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Report Number
DRDC-SUFFIELD-TM-2009-144 — Technical Memorandum
Date of publication
01 Oct 2009
Number of Pages
30
DSTKIM No
CA034703
CANDIS No
534208
Format(s):
Electronic Document(PDF)

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