HEMOGLOBIN-BASED BLOOD SUBSTITUTES: CHARACTERIZATION OF FIVE PYRIDOXAL 5'-PHOSPHATE DERIVATIVES OF HEMOGLOBIN

Authors
  1. McGarrity, M.J.
  2. Er, S.S.
  3. Hsia, J.C.
Corporate Authors
Defence and Civil Inst of Environmental Medicine, Downsview ONT (CAN)
Abstract
Six fraction (I-IV) of pyridoxal 5'-phosphate (PLP) hemoglobin (Hb), prepared by the method of De Venuto and Zegna (J. Surg. Res., 34 (1983) 205) have been isolated and purified by anion-exchange high-performance liquid chromatography (HPLC). Total phosphate analyses indicate that I is HbA, II and III are double-labelled, IV and V are tetra-labelled and VI contains 6 mol of phosphorus per mol of hemoglobin. The purified components have been resolved into their alpha and beta chains by preparative reversed-phase HPLC using a macroporous C4 Support. Phosphate analyses indicate that the beta chains of II, III and IV each contain one phosphate per chain while the beta chains of V and VI each contain two phosphates.
Report Number
DCIEM-87-P-10 — Research Paper; Reprint
Date of publication
01 Jan 1987
Number of Pages
14
Reprinted from
J of Chromatography, no 419, 1987, p 37-50
DSTKIM No
88-00245
CANDIS No
53488
Format(s):
Microfiche filmed at DSIS;Originator's fiche received by DSIS;Hardcopy

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