A Comparison of Suspension Array Immunoassay and Enzyme-Linked Immunosorbant Assay for the Identification of the Toxin Simulant Ovalbumin

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Authors
  1. Bhogal, H.S.
  2. Fulton, R.E.
  3. McLaws, L.J.
  4. Snodgrass, M.
  5. Dickinson-Laing, T.
  6. Mah, D.C.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
A microsphere-based suspension array (SA) system was used for the development and characterization of an immunoassay for the toxin simulant ovalbumin. Results obtained by SA immunoassay were compared with those obtained by enzyme-linked immunosorbent assay (ELISA) using the same immunoreagents. The limit of detection (LOD) for the SA ovalbumin assay was 4.9 ng/mL, compared to a LOD of 0.01 ng/mL for the ovalbumin ELISA. Although the ELISA LOD exceeded that of the SA assay, the SA assay was more simple and rapid to perform, with assays being completed in half the time of the traditional ELISA. The well-to-well reproducibility (coefficient of variation (CV)) of the ELISA and the SA array assay was determined to be 4.9% and 5.1%, respectively. The ELISA and SA array assay plate-plate reproducibility was 14.8% and 6.1%, respectively. The protocols used to develop the SA assay for ovalbumin may be used as a template for development of other assays for toxins, bacteria, and viruses. Subsequently, SA assays for individual biothreat agents may be used as a starting point for the development of multiplexed assays for multiple agents.
Report Number
DRDC-SUFFIELD-TM-2007-261 — Technical Memorandum
Date of publication
01 Dec 2007
Number of Pages
36
DSTKIM No
CA037616
CANDIS No
537303
Format(s):
Electronic Document(PDF)

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