Microchip-based sample processing for bio-analysis and mass spectrometry – Microfluidic systems for protein sample preparation

Authors
  1. Chonn, A.
  2. Jemere, A.
  3. Gong, Z.
  4. Ai, H.
  5. Dragolcic, J.
  6. Oleschuck, R.
  7. Marecak, D.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
Abstract
The detection and identification of pathogenic bacteria and their protein toxins play a crucial role in a proper response to natural or terrorist-caused outbreaks of infectious diseases. Mass spectrometry (MS) has the potentialto obtain partial protein sequence information for the purpose of microorganism identification. However, such MS approaches typically require a number of time-consuming and cumbersome sample preparation steps, namely, extraction of a specific protein to be analyzed from a bacterial extract, enzymatic digestion of the protein, separation of the digested products, clean-up of products by solid phase extraction, and injection of the sample into a mass spectrometer (usually by electrospray).The main goal of this project was to design and develop a microfluidics device prototype for integrated sample preparation and sample introduction for MS analysis. We employed two different technologic approaches: a microfluidic chip-based approach that was developed in the laboratory of Dr. Jed Harrision at the University of Alberta, and a microarray bead-based approach that was developed in the laboratory of Dr. Richard Oleschuk at Queen’s University. We demonstrate the utility of both micro fluidics technologies and microarray technologies in integrating a number of sample preparation steps. We provided a microfluidic chip prototype that was capable of performing affinity capture of proteins, enzyme-based protein digestion, solid phase extraction and subsequent deli

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Report Number
DRDC-SUFFIELD-CR-2012-085 — Contractor Report
Date of publication
01 Jun 2012
Number of Pages
40
DSTKIM No
CA038126
CANDIS No
537928
Format(s):
Electronic Document(PDF)

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