Microchip-based sample processing for bio-analysis and mass spectrometry – Microfluidic systems for protein sample preparation

  1. Chonn, A.
  2. Jemere, A.
  3. Gong, Z.
  4. Ai, H.
  5. Dragolcic, J.
  6. Oleschuck, R.
  7. Marecak, D.
Corporate Authors
Defence R&D Canada - Suffield, Ralston ALTA (CAN)
The detection and identification of pathogenic bacteria and their protein toxins play a crucial role in a proper response to natural or terrorist-caused outbreaks of infectious diseases. Mass spectrometry (MS) has the potentialto obtain partial protein sequence information for the purpose of microorganism identification. However, such MS approaches typically require a number of time-consuming and cumbersome sample preparation steps, namely, extraction of a specific protein to be analyzed from a bacterial extract, enzymatic digestion of the protein, separation of the digested products, clean-up of products by solid phase extraction, and injection of the sample into a mass spectrometer (usually by electrospray).The main goal of this project was to design and develop a microfluidics device prototype for integrated sample preparation and sample introduction for MS analysis. We employed two different technologic approaches: a microfluidic chip-based approach that was developed in the laboratory of Dr. Jed Harrision at the University of Alberta, and a microarray bead-based approach that was developed in the laboratory of Dr. Richard Oleschuk at Queen’s University. We demonstrate the utility of both micro fluidics technologies and microarray technologies in integrating a number of sample preparation steps. We provided a microfluidic chip prototype that was capable of performing affinity capture of proteins, enzyme-based protein digestion, solid phase extraction and subsequent deli

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Report Number
DRDC-SUFFIELD-CR-2012-085 — Contractor Report
Date of publication
01 Jun 2012
Number of Pages
Electronic Document(PDF)

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