SENSITIVE RAPID DETECTION METHOD FOR VIABLE BACTERIAL CELLS

Authors
  1. MacLeod, R.A.
  2. Light, M.
  3. White, L.A.
  4. Currie, J.F.
Corporate Authors
Defence Chemical Biological and Radiation Labs, Ottawa ONT (CAN)
Abstract
A rapid sensitive method for the detection of viable bacterial cells is described in which P32 as inorganic orthophosphate is used to label the cells. Factors affecting the uptake of P32 by cells as well as the sensitivity of the method have been explored with suspensions of Aerobacter aerogenes. The uptake of P32O4 is dependent on several factors. Of various incubation media tested, one composed of 0.005 M KC1, 0.002 m MgSO4 and 10 mg/ml of glucose was found to best stimulate the uptake of the tracer. Incubation time and temperature and level of isotope and of unlabeled P also affected uptake. Labeled cells were collected on a membrane filter for measurement of radioactivity. Under optimal conditions, as few as 23 viable cells per milliliter were detected in 1 hr with 95% confidence.
Keywords
Aerobacter aerogenes
Report Number
DCBRL-507 — Reprint; Previously Catalogued as DSIS 67-02920
Date of publication
15 Nov 1966
Number of Pages
7
Reprinted from
Applied Microbiology, vol 14, no 6, 1966, p 979-984
DSTKIM No
89-01270
CANDIS No
59202
Format(s):
Hardcopy;Originator's fiche received by DSIS

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