DEVELOPMENT OF ELISA TECHNIQUES FOR THE DETECTION AND IDENTIFICATION OF ADENOVIRUS INFECTION

Authors
  1. Bishai, F.R.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN);McGill Univ, Montreal QUE (CAN)
Abstract
An enzyme linked immunosorbent assay (ELISA) has been developed for the detection of adenoviruses and their antibodies in patients' specimens. The developed ELISA for the detection of antibodies to adenoviruses is approximately 500 times more sensitive than the CF test which is presently used in many diagnostic laboratories for the detection of adenovirus infection in patients' sera. The developed ELISA can also detect up to one TCID sub 50 of adenovirus in a specimen. We have also identified the specific viral proteins (antigens) for each of adenovirus types 4 and 7a by sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE) and Western Blot Techniques. These results have been utilized in establishing a new immunoblotting assay for the detection and identification of specific antibodies to adenovirus Types 4 and 7a in sera collected from patients and/or vaccinees' sera.
Report Number
DRES-CR-5/89 — Contract Report (Final)
Date of publication
15 Apr 1988
Number of Pages
40
DSTKIM No
89-02229
CANDIS No
60135
Format(s):
Hardcopy;Originator's fiche received by DSIS

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