SPECIES SPECIFIC EFFECT OF OMEGA-CONOTOXIN ON INTRACELLULAR CALCIUM LEVELS

Authors
  1. Hamilton, M.G.
  2. Lee, W.E.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
Omega-Conotoxin GVIA (CgTx), a component of the venom of the piscivorus snail Conus geographus is an extremely potent inhibitor of Ca++ channels. Significant inhibition of voltage stimulated Ca++ channel (VSCC) influx has been reported at micromolar and lower concentrations. We have examined the effects of this toxin on the regulation of intracellular Ca++ levels in rat and chicken brain synaptosomes and in primary cultures of mouse cortical neurones. The intracellular concentration of free ionized Ca++ was monitored using the Ca++ sensitive dye, FURA-2. With this technique, very small changes in free intracellular Ca++ levels were easily detected. Ca++ concentrations in synaptosomes from either chicken or rat synaptosomes or mouse cortical neurons were not significantly different from each other. Similarly, the response of these neuronal preparations to depolarizing stimuli (K+) was very similar. TRUNCATED
Report Number
DRES-522 —
Date of publication
15 May 1989
Number of Pages
23
DSTKIM No
89-02810
CANDIS No
60714
Format(s):
Hardcopy;Originator's fiche received by DSIS

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