A METHOD FOR THE QUANTITATION OF TRACE LEVELS OF DIMETHYL SULFOXIDE IN URINE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Authors
  1. Chenier, C.L.
  2. Hancock, J.R.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
A semi-automated liquid chromatographic system for the extraction and concentration of trace levels of dimethyl sulfoxide (DMSO) in human urine has been developed. Urine samples are passed through a low pressure cation-exchange column as a cleanup step prior to solid phase extraction on a low pressure charcoal column. DMSO is eluted from the charcoal column with acetonitrile and after separation by reversed-phase high performance liquid chromatography, is quantified by ultra-violet detection at a wavelength of 196nm. The recovery of DMSO from the urine is 82% + or - 7% (n=36) with a detection limit of 0.1 mu g/mL in urine. With this method, previously unreported low levels of naturally occurring DMSO were discovered. The method also revealed a decrease in recoveries of DMSO with time indicating a potential instability of the analyte at these low concentrations.
Report Number
DRES-495 —
Date of publication
15 May 1989
Number of Pages
31
DSTKIM No
89-03075
CANDIS No
60976
Format(s):
Hardcopy;Originator's fiche received by DSIS

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