LONG TERM STORAGE OF ANTIBODY-SENSITIZED MILLITITER™ IMMUNOASSAY PLATES FOR THE IDENTIFICATION AND QUANTITATION OF FRANCISELLA TULARENSIS

Authors
  1. Siddiqui, Y.M.
  2. Fulton, R.E.
Corporate Authors
Defence Research Establishment Suffield, Ralston ALTA (CAN)
Abstract
The report describes a modified procedure for the "sandwich" fluorogenic enzyme-linked immunosorbent assay (FELISA) for the detection and quantitation of antigen, in which immunoassay plates were pre-coated with capture antibody or, alternatively, with capture antibody and blocking reagent, and stored frozen for periods up to 1 yr. Using Francisella tularensis as a model, two different identification protocols were employed. In the first, antibody-sensitized immunoassay plates stored frozen at -70C were challenged with whole cells or with outer membrane protein of F. tularensis. In these experiments, there was no loss of sensitivity for the detection and quantitation of the antigen, when compared with freshly prepared immunoassay plates. This procedure took 5 h compared to more than 6 h required for performance of the assay using freshly prepared plates. TRUNCATED
Report Number
DRES-M-1282 — Memorandum
Date of publication
15 Aug 1989
Number of Pages
19
DSTKIM No
89-03847
CANDIS No
61741
Format(s):
Hardcopy;Originator's fiche received by DSIS

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